| Registro completo |
| Provedor de dados: |
Rev. Bras. Frutic.
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| País: |
Brazil
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| Título: |
A microsatellite library for Carica papaya L. cv. Sunrise solo
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| Autores: |
Santos,Silvia Correa
Ruggiero,Carlos
Silva,Cristina Lacerda Soares Petrarolha
Lemos,Eliana Gertrudes Macedo
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| Data: |
2003-08-01
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| Ano: |
2003
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| Palavras-chave: |
Carica papaya
Sex
Microsatellite markers
Library
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| Resumo: |
In experimental areas of the Education and Researches Ilha Solteira and Jaboticabal UNESP/Campus farms were selected and tagged 20 hermaphrodite plants and 20 feminine of cultivar Sunrise Solo, Improved Sunrise Solo cv.72/12 and Baixinho of Santa Amália.The seeds origined of the selected fruits were cropped to be analysed the self-pollination efficiency and frequency of the sex in the progenies. After that, samples of the young leaf of the matrix plants were colected for the extration of the DNA. It was built five library enriched of microsatellite sequencies, using probes (TCA)10, (TC)13, (GATA)4, (CAC)10 e (TGAG)8.It was possible the development of the primers only in the library that has utilized the probe (TCA)10. This probe allowed the design of 32 primer pairs. From these, 31 presented pattern of unique band in agarose Metaphor and in acrilamide. For primer S36 were observed 2 bands, but with no polymorphism to differentiation in the sexual form at papaya tree culture. However, these primers can be tested, in the futures, in the investigation of the others features in segregated populations of this specie and the related species, germoplasm analysis, cultivars identification, parent evolution and molecular markers for the assisted plant breeding programs.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000200020
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| Editor: |
Sociedade Brasileira de Fruticultura
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| Relação: |
10.1590/S0100-29452003000200020
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| Formato: |
text/html
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| Fonte: |
Revista Brasileira de Fruticultura v.25 n.2 2003
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| Direitos: |
info:eu-repo/semantics/openAccess
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